Various mushrooms which have been widely used in Korea, China and Japan as a health enhancer and ancient herbal medicine. A variety of mushrooms have been used traditionally in different cultures for the maintenance of health and in the prevention and treatment of various diseases [1]. The medicinal mushroom is used as a folk medicine in the Western Ghats of India. Fungal metabolites or other related compounds could be used to develop neutraleutic products or drugs effective against pathogenic microorganisms resistant to conventional treatments. medicinal mushrooms contains various bioactive substances, including polysaccharides and proteoglycans, which have been shown to exert various properties such as antitumor, immunomodulatory, antioxidant, radical scavenging, cardiovascular, antihypercholesterolemic, antiviral, antibacterial, antiparasitic, antifungal, detoxifying, hepatoprotective and antidiabetic. effects[2].The antibacterial activity of medicinal mushrooms describes the action of its extracts without identifying the compounds responsible for this activity. Mushrooms have shown antimicrobial activity against pathogenic microorganisms, including bacteria associated with nosocomial infections (Pseudomonas aeruginosa, Pseudomonas maltophila, Listeria monocytogenes, Staphylococcus aureus, Klebsiella pneumoniae, Morganella morganii, Proteus mirabilis, Serratia marcescens, Escherschia coli, Aceinetobacter) and multidrug resistance. Multiple drug resistance has developed in human pathogenic microorganisms due to the indiscriminate use of commercial antimicrobial drugs [3]. Increasing bacterial resistance to antibiotics and antimicrobials is a growing concern for the medical, food, and sanitation industries [4]. Among the numerous fungi, Ph...... half of the paper ......f cork borer (6 mm) and extracts (80μ/l) were added to each well. The plates were incubated at 35-37°C for 18-24 hours, the activity was calculated by measuring the diameter of the inhibition zone and the results were compared and analysed. Minimum Inhibitory Concentration (MIC) Analysis The MIC of Phellinus samples was performed by the broth dilution method. A series of tubes containing 9 ml of brothy medium was prepared, a known concentration of Phellinus sample extract was added to the first tube and the solution was mixed thoroughly. A serial dilution was performed and after dilution 10μl/ml of the microbial test suspension was added to each tube, including the control tubes. The tubes were incubated at 37°C for 18-24 hours. The tubes were observed for the growth of microorganisms. The concentration of Phellinus sample extracts ranged from 2 μl/ml to 1024 μl/ml [5,10].