The calla lily (Zantedeschia spp.), a genus of the Araceae family, is a perennial flowering plant and grows naturally in central and southern Africa (Wei et al. , 2017). This genre is composed of two sections. The first group is the Zantedeschia aethiopica called white calla, equipped with a rhizomatous storage organ and is evergreen. The second group is represented by the colorful calla lilies which have a tuberous storage organ and require a dormant period (Zhang et al., 2011). Calla lilies are commercially available as cut flowers and potted plants. These flowers have a high commercial value on the market due to their attractive inflorescence (Jonyviene et al., 2017). According to statistics from 2014, the turnover of calla lilies was €19 million (euros) in the Netherlands (Floraholland facts and figures, 2014). It contributed a substantial part of the horticultural export earnings to the Netherlands and America. The yield of colored calla tubers in these two countries has increased significantly in recent years to meet foreign market demand, especially in Asia (Wei et al., 2017). In most cases, conventional propagation of calla lilies consists in dividing the tuber, but this method requires high skills and also provokes the development of the disease soft root rot. Bacterial soft rot disease caused by Erwinia carotovora subsp. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an original essayCarotovora is a big problem in Zantedeschia spp. Infection occurs at all stages of the life cycle, from the time of plant growth in the field to tubers in storage, and spreads rapidly in calla lilies (Wright et al., 2002). The final stage is the destruction of the plant and death; losses can approach 100% in a severe outbreak. For a long time, this disease has been recognized as a major cause and fatal disease on which the calla lily industry has not made progress (Cho et al., 2013). It should be noted that the acquisition of vigorous plants is considered essential for satisfactory floral production. Knowledge and application of principles and techniques from choice of propagation material to seedling management are necessary to produce high-quality plants (Oliveira, 2007). Therefore, growers are looking for a way to reduce problems and produce high-quality calla plants. The use of in vitro culture methods can be effective in reducing losses and increasing quality (Cheng et al., 2003). Propagation via tissue culture is the most suitable method to quickly obtain numerous pathogen-free plants (Kulpa, 2016). In fact, micropropagation is widely used for commercial purposes as a method of vegetative propagation from different explants. The successful use of tissue culture techniques has been announced in several reports for the rapid propagation of the Zantedeschia genus. Chang et al (2003) presented a method for micropropagation of Zantedeschia albomaculata using shoot tip proliferation. The results showed that 6-benzyladenine (BA) and thidiazuron (TDZ) were more effective, and the optimal concentration of BA or TDZ was 8.87 mM and 4.54 mM, respectively. Koech et al (2005) described a protocol for the in vitro regeneration of shoots of Zantedeschia albomaculata, using explants of shoot primordia. Shoot explants can be used to produce multiple high-quality shoots using MS medium supplemented with 2.0 mg L-1 BAP. In their experiment, no response was observed in both leaf and tuber explants. Duquenne.